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TOOLSmart RNA Extractor

Data sheet MSDS
TOOLSmart RNA Extractor is developed based on regular nucleic acid extraction Reagent with the addition of indicator. It has better lysis ability and higher sensitivity. It could be used to isolate total RNA from samples like virus, bacteria, plant tissue and body fluid. It maintains the integrity of RNA while disrupting cells and dissolving cell components during sample homogenization. TOOLSmart RNA Extractor can be applied to isolate RNA from both small amount of samples (50-100 mg tissue or 5 × 106cells) and large amount of samples (≥1 g tissue or ≥107cells) of human, animal, plant or bacteria, within one hour. DNA and protein contamination is eliminated thoroughly from RNA products. Isolated RNA can be used in Northern Blot, Dot Blot, poly(A) selection, in vitro translation, RNase protection assay and molecular cloning.

 

No. Size Price Qty
DPT-BD24 100ml x2 $226.67
The price does not include shipping fee and tax. ORDER
Features
  • High yield, rapid purification of high-quality RNA.
  • Purify RNA within 1 hour.
  • Suitable for any sample type.
  • Low sample amount limitation.
  • Retain short RNA fragment.
  • High sensitivity, suitable for virus RNA extraction.
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Work Flow

                              

      Total RNA extraction result

    

     Fig 1. RNA extracted from rat liver tissue

     using 1ml of TOOLSmart RNA Extractor. 

     Sample amount: 30mg of rat liver.

     3ul of 80ul eluted RNA were leaded per lane.

 

 

    

     Fig 2. RNA extracted from HepG2 cells

     using 1ml of TOOLSmart RNA Extractor. 

     Sample amount: 1x10^6 HepG2 cells.

     3ul of 30ul eluted RNA were leaded per lane.

 

 

    

    Fig 3. RNA extracted from Oryza sativa

    leaves using 1ml of TOOLSmart RNA 

    Extractor. Sample amount: 100mg.

    3ul of 50ul eluted RNA were loaded per lane.


    miRNA extraction result


    

   Fig 4. miRNA extracted from U937
    cells (8 x 10^6 cells)
    lane1: 100bp marker
    lane2: other brand
    lane3: TOOLSmart RNA Extractor

  1. Chen CY, Chen CC, Chuang WY, Leu YL, Ueng SH, Hsueh C, Yeh CT, Wang TH. Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells. Front Oncol. 2020 Feb 25;10:216. 

  2. Huang CS, Ho JY, Chiang JH, Yu CP, Yu DS. Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells. Cells. 2020 Jun 7;9(6):1419. 

  3. Lin CY, Chao A, Wu RC, Lee LY, Ueng SH, Tsai CL, Lee YS, Peng MT, Yang LY, Huang HJ, Wang HS, Lai CH. Synergistic effects of pazopanib and hyperthermia against uterine leiomyosarcoma growth mediated by downregulation of histone acetyltransferase 1. J Mol Med (Berl). 2020 Aug;98(8):1175-1188. 

  4. Lin CY, Lee LY, Wang TH, Hsu CL, Tsai CL, Chao A, Lai CH. Palbociclib Promotes Dephosphorylation of NPM/B23 at Threonine 199 and Inhibits Endometrial Cancer Cell Growth. Cancers (Basel). 2019 Jul 20;11(7):1025. 

  5. Wang TH, Leu YL, Chen CC, Shieh TM, Lian JH, Chen CY. Psorachromene Suppresses Oral Squamous Cell Carcinoma Progression by Inhibiting Long Non-coding RNA GAS5 Mediated Epithelial-Mesenchymal Transition. Front Oncol. 2019 Nov 5;9:1168. 

  6. Chou LF, Chen CY, Yang WH, Chen CC, Chang JL, Leu YL, Liou MJ, Wang TH. Suppression of Hepatocellular Carcinoma Progression through FOXM1 and EMT Inhibition via Hydroxygenkwanin-Induced miR-320a Expression. Biomolecules. 2019 Dec 21;10(1):20. 

  7. Chen HF, Hsu CM, Huang YS. CPEB2-dependent translation of long 3'-UTR Ucp1 mRNA promotes thermogenesis in brown adipose tissue. EMBO J. 2018 Oct 15;37(20):e99071. 

  8. Chen CY, Fang JY, Chen CC, Chuang WY, Leu YL, Ueng SH, Wei LS, Cheng SF, Hsueh C, Wang TH. 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression. Front Oncol. 2020 Aug 11;10:1319.

  9. Lin H, Huang YS, Fustin JM, Doi M, Chen H, Lai HH, Lin SH, Lee YL, King PC, Hou HS, Chen HW, Young PY, Chao HW. Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liver. Nat Commun. 2021 Jan 28;12(1):645.

  10. Chu CY, Lee YC, Hsieh CH, Yeh CT, Chao TY, Chen PH, Lin IH, Hsieh TH, Shih JW, Cheng CH, Chang CC, Lin PS, Huang YL, Chen TM, Yen Y, Ann DK, Kung HJ. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics. 2021 Jan 25;11(8):3624-3641.

  11. Wu YS, Ho JY, Yu CP, Cho CJ, Wu CL, Huang CS, Gao HW, Yu DS. Ellagic Acid Resensitizes Gemcitabine-Resistant Bladder Cancer Cells by Inhibiting Epithelial-Mesenchymal Transition and Gemcitabine Transporters. Cancers (Basel). 2021 Apr 22;13(9):2032.

  12. Lin CY, Tsai CL, Chao A, Lee LY, Chen WC, Tang YH, Chao AS, Lai CH. Nucleophosmin/B23 promotes endometrial cancer cell escape from macrophage phagocytosis by increasing CD24 expression. J Mol Med (Berl). 2021 Aug;99(8):1125-1137.

  13. Wu SC, Chang CH, Chang LH, Wu CW, Chen JW, Chen CH, Lin YS, Chang JK, Ho ML. Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment. Biomedicines. 2021 May 17;9(5):559.

  14. Lin YH, Wu MH, Liu YC, Lyu PC, Yeh CT, Lin KH. LINC01348 suppresses hepatocellular carcinoma metastasis through inhibition of SF3B3-mediated EZH2 pre-mRNA splicing. Oncogene. 2021 Jul;40(28):4675-4685.

  15. Chang LH, Chen CH, Wu SC, Chang JK, Ho ML. Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats. J Orthop Translat. 2021 Aug 12;30:16-30.

  16. ​Huang SW, Shen MC, Wang WH, Li WY, Wang JH, Tseng CY, Liu PY, Wang LS, Lee YL, Chen YA, Lee CY, Lu PL, Wang SF. High prevalence of HIV-1 transmitted drug resistance and factors associated with time to virological failure and viral suppression in Taiwan. J Antimicrob Chemother. 2021 Oct 14:dkab361.

  17. ​Chiang KY, Li YW, Li YH, Huang SJ, Wu CL, Gong HY, Wu JL. Progranulin A Promotes Compensatory Hepatocyte Proliferation via HGF/c-Met Signaling after Partial Hepatectomy in Zebrafish. Int J Mol Sci. 2021 Oct 18;22(20):11217.

  18. ​Chen CC, Chen CY, Cheng SF, Shieh TM, Leu YL, Chuang WY, Liu KT, Ueng SH, Shih YH, Chou LF, Wang TH. Hydroxygenkwanin Increases the Sensitivity of Liver Cancer Cells to Chemotherapy by Inhibiting DNA Damage Response in Mouse Xenograft Models. Int J Mol Sci. 2021 Sep 9;22(18):9766.

  19. ​Huang CS, Tsai CH, Yu CP, Wu YS, Yee MF, Ho JY, Yu DS. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel). 2022 Feb 15;14(4):968.

  20. ​Hung CT, Su TH, Chen YT, Wu YF, Chen YT, Lin SJ, Lin SL, Yang KC. Targeting ER protein TXNDC5 in hepatic stellate cell mitigates liver fibrosis by repressing non-canonical TGFβ signalling. Gut. 2021 Dec 21:gutjnl-2021-325065.

Q1. What is the amount of tissue that can be extracted with 200ml of RNA Extractor?
A: 1ml RNA Extractor can extract 20-50mg tissue.

Q2. Can RNA Extractor extract miRNA?
A: Yes. RNA Extractor can extract total RNA (including miRNA).

Q3. Is there any recommended kit for extracting RNA from fat, muscle, liver ,cells?
A: Toolsmart RNA extractor is the best choice.

Q4. Do you suggest this extractor with only 10^4 cells?
A: Not recommended. But if your number of cells can only be that much, you can try and need to be careful in the wash step to avoid any more RNA loss